<i>In Vitro </i>Fungitoxic Activity of Some Glucosinolates and Their Enzyme-Derived Products toward Plant Pathogenic Fungi

Abstract

The enzymatic hydrolysis of glucosinolates (GLs), typical compounds of Cruciferae, produces molecules with fungitoxic activity. Eleven GLs and their enzymatic hydrolysis products obtained by myrosinase were tested in vitro against Fusarium culmorum. Toxicity of hydrolysis products from glucoiberin, glucotropaeolin, sinigrin, and epiprogoitrin were assayed on eight plant pathogenic fungi. The results showed (i) the native GLs showed no fungitoxic activity, whereas their hydrolysis products inhibited fungal growth depending on their chemical structure; (ii) the hydrolysis products from glucoiberin, glucoerucin, glucocheirolin, and glucotropaeolin were the most effective, with 50% inhibition of fungal growth at 0.1 mg/mL; (iii) the fungitoxic activity of hydrolysis products obtained from glucoiberin, glucotropaeolin, sinigrin, and epiprogoitrin was confirmed on eight pathogenic fungi, with different responses depending on their chemical structure; (iv) the most effective hydrolysis products were those from glucoiberin, showing EC 50 values of 0.05 mg/mL on Rhizoctonia solani, Sclerotinia sclerotiorum, Diaporthe phaseolorum, and Pythium irregulare and a minimum inhibitory concentration varying from 0.1 to 1.2 mg/mL. Keywords: Cruciferae; isothiocyanates; Fusarium culmorum; myrosinase