Journal of Food Science · 1986 · 60 citations · 9 references
AgricultureFood ScienceMicrobiologyFood AnalysisFood MicrobiologyFood SafetyAntigen Detection AssaysEnzyme-linked Immunosorbent AssayFungal DiagnosticsMolds In FoodsPathogen DetectionPathogen Detection In FoodsAnalytical Detection LimitsDetection Of Pathogens In VegetablesImmunoassay Cross-reactivityImmunoassays For Food AllergensMold AllergensMold Contamination In FoodsMold IdentificationMolds On Vegetables
ABSTRACT A double‐sandwich enzyme‐linked immunosorbent assay (ELISA) was developed for the detection of molds ( Ahernaria alternata, Geotrichum candidum and Rhizopus stolonifer ) in tomato puree by the use of antisera raised in rabbits injected with homogenates of the lyophilized boiled molds. Cross reactivity among the three species was less than 10%. Detection limits were approximately 1μg dried mold/g of sample, a sensitivity greater than that of most chemical methods. Positive relationships between ELISA readings and the amounts of mold added to puree were observed, while background ELISA values for puree controls were negligible.
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Annals of Internal Medicine · 1968 · 2.4K citations · Full text
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Annals of Internal Medicine · 1977 · 205 citations