An effective family shuffling method using single-stranded DNA

[1] Production of single-stranded plasmid DNA

Jeffrey Vieira, Joachim Messing · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 2.6K citations

Life Sciences, Biotechnology, Molecular Biology +12

Rapid evolution of a protein in vitro by DNA shuffling

Willem P.C. Stemmer · Nature · 1994 · 1.9K citations

Life Sciences, Genetics, Molecular Biology +10

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

Willem PC Stemmer · Proceedings of the National Academy of Sciences · 1994 · 1.3K citations

Life Sciences, Biology, Molecular Biology +15

Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

Andreas Crameri, Erik A. Whitehorn, Emily Tate et al. · Nature Biotechnology · 1996 · 1.2K citations

Life Sciences, Biotechnology, Molecular Biology +11

DNA shuffling of a family of genes from diverse species accelerates directed evolution

Andreas Crameri, Sun-Ai Raillard, Ericka Bermudez et al. · Nature · 1998 · 828 citations

Life Sciences, Genetics, Molecular Biology +13