Proceedings of the National Academy of Sciences · 1996 · 37 citations · 31 references
Life SciencesGeneticsMolecular BiologyDna RepairImmunogeneticsRecombinationAntigen Receptor Gene RearrangementRecombination InitiationRecombination IntermediatesRecombination ProteinsV(d)j RecombinationChromosomal InversionsRag1-mediated RecombinationReceptorsRecombination Signal Sequence RecognitionRecombination Signal SequencesSignal FormationSignal Joint FormationV(d)j Recombination Mechanism
The first step of V(D)J recombination, specific cleavage at the recombination signal sequence (RSS), can be carried out by the recombination activating proteins RAG1 and RAG2. In vivo, the cleaved coding and signal ends must be rejoined to generate functional antigen receptors and maintain chromosomal integrity. We have investigated signal joint formation using deletion and inversion substrates in a cell free system. RAG1 and RAG2 alone or in combination were unable to generate signal joints. However, RAG1 and RAG2 complemented with nuclear extracts were able to recombine an extrachromosomal substrate and form precise signal joints. The in vitro reaction resembled authentic V(D)J recombination in being Ku-antigen-dependent.
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pEF-BOS, a powerful mammalian expression vector
Seiichi Mizushima, Shigekazu Nagata · Nucleic Acids Research · 1990 · 1.6K citations · Full text
RAG-1 and RAG-2, Adjacent Genes That Synergistically Activate V(D)J Recombination
Marjorie A. Oettinger, David G. Schatz, Carolyn Gorka et al. · Science · 1990 · 1.5K citations
The V(D)J recombination activating gene, RAG-1
David G. Schatz, Marjorie A. Oettinger, David Baltimore · Cell · 1989 · 1.2K citations · Full text