Mammalian expression and hollow fiber bioreactor production of recombinant anti-CEA diabody and minibody for clinical applications

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251K citations

Protein Assembly, Natural Sciences, Structural Proteins +8

Molecular cloning: A laboratory manual

Prescott L. Deininger · Analytical Biochemistry · 1990 · 86.2K citations

Molecular Biotechnology, Natural Sciences, Genetics +8

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Immunocytochemical Technique, Glycobiology, Polyacrylamide Gels +19

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

Robert M. Horton, Henry D. Hunt, Steffan N. Ho et al. · Gene · 1989 · 3.1K citations

Engineering, Hybridisation, Hybridization +13

Protein chromatography on calcium phosphate columns

Arne Tiselius, Stellan Hjertén, Östen Levin · Archives of Biochemistry and Biophysics · 1956 · 1.5K citations

Biochemistry, Bioanalysis, Calcium Phosphate Columns +5