ChemBioChem · 2012 · 83 citations · 26 references
Life SciencesBiochemistryMolecular BiologyBioorthogonal ChemistryEnzymologyProteomicsActivity-based Protein ProfilingChemical Probe DiscoveryEnzyme ScreeningFunctional ProteomicsMass Spectrometry-based ProteomicsProteolytic EnzymesActivity-based Probe LibrariesClick ChemistryClick Chemistry LabelingMass Spectrometry ProteomicsPeptide Mass SpectrometryProtease Activity MappingProtease AssaysProtease Discovery
Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.
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