Photochemistry and Photobiology · 1994 · 48 citations · 13 references
Life SciencesPlant BiologyPlant SciencesPhotosynthesisPlant BiochemistrySpectroscopyAbsorption In TissueChloroplast BiologyChloroplast StructureChloroplast UltrastructureLeaf PhysiologyPhotosynthetic PigmentsAbsorption SpectrumAbsorption Spectrum CalculationChlorophyll Content MeasurementLeaf Optical PropertiesLeaf Spectral PropertiesLight Ray PropagationMultiple ScatteringPhotosynthetically Active Radiation
Abstract On the basis of a comprehensive treatment of light propagation in plant tissue the first authentic in vivo absorption spectrum of chlorophyll in a leaf (of Catalpa bignonioides ) has been elucidated. An experimental‐theoretical procedure is described, which permits derivation of the authentic chlorophyll spectrum by purifying the in vivo absorption spectrum of a leaf from the distortions of optical origin: multiple scattering and distributional error. The procedure involves measurements of internal and external light fluxes, spatial characterization of tissue layers, cells, chloroplasts and grana stacks. Furthermore, one has to determine the local chlorophyll concentrations. The theories of radiation transfer and absorption statistics make up the theoretical basis of the procedure. The derived authentic chlorophyll spectrum differs both from the in vivo spectrum of the leaf and (although to a smaller degree) from the in vitro spectrum of chlorophyll‐protein complexes extracted from the same leaf.
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Plants and the daylight spectrum.
Helen Smith · 1981 · 745 citations
Leonid Fukshansky, Alexander Martinez v. Remisowsky, John H. McClendon et al. · Photochemistry and Photobiology · 1993 · 105 citations