A new cloning strategy for generating multiple repeats of a repetitive polypeptide based on non-template PCR

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

Hermann Schägger, Gebhard von Jagow · Analytical Biochemistry · 1987 · 11.5K citations

Life Sciences, Biochemistry, Molecular Biology +10

Genetically Encoded Synthesis of Protein-Based Polymers with Precisely Specified Molecular Weight and Sequence by Recursive Directional Ligation:  Examples from the Elastin-like Polypeptide System

Dan E. Meyer, Ashutosh Chilkoti · Biomacromolecules · 2002 · 551 citations

Engineering, Bioengineering, Biotechnology +17

Characterization of a Helical Protein Designed from First Principles

Lynne Regan, William F. DeGrado · Science · 1988 · 551 citations

Life Sciences, Biochemistry, Molecular Biology +17

Dodecamer repeat expansion in cystatin B gene in progressive myoclonus epilepsy

Maria D. Lalioti, Hamish S. Scott, Catherine Burési et al. · Nature · 1997 · 358 citations

Life Sciences, Genetics, Neurology +16

Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR

Jan Košťál, Rosanna Yang, Cindy H. Wu et al. · Applied and Environmental Microbiology · 2004 · 228 citations · Full text

Engineering, Bioengineering, Biotechnology +15