Journal of Agricultural and Food Chemistry · 1997 · 48 citations · 32 references
PharmacyBiochemistryToxicologyAntibody EngineeringMonoclonal AntibodiesPesticide ToxicologyAntibody CharacterizationAntibody ConjugatesAntibody ConjugationAntibody ScreeningInsecticide ToxicologyPesticide ChemistryAnalytical Characterization Of ConjugatesAnalytical Methods DevelopmentHapten ImmunoassaysInsecticide ChemistryOrganochlorine CompoundsOrganochlorine PesticidesPesticide DetectionSelection Criterion Design
This work describes the production and characterization of monoclonal antibodies (MAbs) to the organochlorine insecticide DDT and their incorporation into several ELISA configurations. A collection of DDT haptens was synthesized by introducing appropriate spacers at two sites of the analyte molecular structure. From mice immunized with hapten−protein conjugates, MAbs with I 50 values to p,p ‘-DDT in the 2−11 nM range in homologous conjugate-coated assays were obtained. According to their cross-reactivity pattern with DDT isomers and metabolites, MAbs can be classified as class-specific or DDT-specific antibodies. Both types of MAbs were obtained from mice immunized with the same hapten−protein conjugate simply by applying a different selection criterion in the screening of fusion supernatants. These immunoassays are potentially very valuable analytical tools for the rapid and sensitive determination of DDT and congeners in food and the environment and for monitoring human exposure to these ubiquitous and toxic compounds. Keywords: ELISA; insecticide; organochlorine pesticide; monoclonal antibodies; DDT; DDE; DDD; hapten design; hapten heterology; ELISA format
32
Eline P. Meulenberg, Wim H. Mulder, Peter G. Stoks · Environmental Science & Technology · 1995 · 162 citations