Growth of Chick Embryos in Polyethylene Bags

Abstract

For the past several years we have attempted to grow chick embryos in artificial membranes. It was our hope to achieve development of chicks to hatching, as a preliminary to creating our own double yolked eggs for immunogenetic studies. Our results while not complete are worthy of mention to others. Hens eggs have been incubated in polyethylene bags and also in plastic chambers using a General Electric silicone membrane. Several types of commercial polyethylene bags were experimented with and the commercial “Baggies” were found to produce the best results. The procedure was simply to place a “Baggie” in a tall beaker with the top of the bag turned back over the rim. The egg was then cracked and emptied into the “Baggie,” which was then lifted out and the top twisted and secured in a loop with a rubber band. The loop provided a hanger for suspending the assembly on…