The Journal of Experimental Medicine · 1915 · 16 citations · 0 references
Veterinary MedicineInfectious DiseasesMicrobiologyDiagnostic MicrobiologyImmunology Of Infectious DiseasesMedical MicrobiologyBacterial InfectionsHost Defense Against InfectionProtective ImmunityAntiserum PreparationHumoral Immunity To BacteriaPneumococcal DiseaseProtein Content DeterminationSerum Precipitating Antibodies
The addition of a water-clear extract of pneumococci to homologous antiserum produces a voluminous precipitate which carries down with it the agglutinins and practically the totality of the protective bodies against pneumococcus infection in animals. This precipitate when washed and resuspended in saline solution to the original volume of serum protects as well as the whole serum. The protein content of such solutions has varied from 0.09 to 0.34 per cent., as contrasted with about 6 per cent. in the original serum. The solution of this precipitate is not necessary to insure protection, and when produced by dilute alkali (sodium hydrate) frequently destroys the immune bodies.