Piezoelectric Biosensor for Detection of <i>Salmonella typhimurium</i>

Abstract

ABSTRACT A flow injection analysis (FIA) system was developed based on a piezoelectric biosensor for detection of Salmonella typhimurium. The anti‐ Salmonella spp antibody was immobilized onto the gold electrode coated quartz crystal surface through a polyethylenimine‐glutaraldehyde (PEG) technique and dithiobis‐succinimidyl propionate (DSP) coupling. The PEG technique proved more successful for FIA applications than the DSP coupling. The biosensor had responses of 23–7 Hz in 25 min when the PEG immobilization technique was employed, with R 2 0.94 for Salmonella typhimurium concentrations of 5.3.10 5 to 1.2.10 9 CFU/mL.