Journal of the American Chemical Society · 2007 · 141 citations · 11 references
Natural SciencesBiochemistryBioengineeringBiomaterialsStructural BiologySupramolecular ChemistryMacromolecular AssemblyMacromolecular StructurePeptide ChemistryProtein StructureProtein-based BiomaterialsSupramolecular MaterialsAromatic Stacking AssembliesAromatic Stacking InteractionsMolecular Recognition ElementsMotif-based Molecular RecognitionPeptide Self-assemblyPeptide-based BiomaterialsSelf-assembling Peptide MaterialsStructural Motif Recognition
We have designed, synthesized, and characterized a short (32-mer; 8-nm), single-stranded, collagen-related peptide (CRP), 1a, which forms triple-helical building blocks that self-assemble into large, composite fibrils by strictly noncovalent means. Computational analysis suggested that the installation of complementary, aromatic π-stacking recognition elements at the N- and C-termini of (Gly-Pro-Hyp) 10 would facilitate the head-to-tail assembly of triple-helical subunits. Our CD, 1 H NMR, DLS, and TEM results for 1a support the formation of such triple-helical, supramolecular structures. Consistent with self-assembly into micrometer-size, composite fibrils, 1a induced the aggregation of human platelets with nearly the same potency as native Type I collagen. The aromatic−aromatic recognition motif employed in this study provides a straightforward approach to collagen-mimetics and has important implications for the design of triple-helical building blocks that can spontaneously oligomerize into functional fibrillar structures.
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Crystal and Molecular Structure of a Collagen-Like Peptide at 1.9 Å Resolution
Jordi Bella, Mark Eaton, Barbara Brodsky et al. · Science · 1994 · 1.1K citations
L F Morton, Philip G. Hargreaves, Richard W. Farndale et al. · Biochemical Journal · 1995 · 320 citations · Full text