Concepedia

Cloning of a wide-spectrum amidase from Bacillus stearothermophilus BR388 in Escherichia coli and marked enhancement of amidase expression using directed evolution☆

Tae Kyou Cheong, Patrick Oriel

Enzyme and Microbial Technology · 2000 · 31 citations · 20 references

DOI

Concepts

References

20

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

Mark A. Bradford · Analytical Biochemistry · 1976 · 225.4K citations

, , +7

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford · Analytical Biochemistry · 1976 · 211.2K citations

, , +7

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

, , +10

Strategies for optimizing heterologous protein expression in Escherichia coli

Gerhard Hannig, Savvas C. Makrides · Trends in biotechnology · 1998 · 489 citations

, , +14

Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

Jeffrey C. Moore, Frances H. Arnold · Nature Biotechnology · 1996 · 424 citations

, , +15