Tetrabutylammonium Fluoride‐Mediated Rapid Alkylation Reaction in Microtiter Plates for the Discovery of Enzyme Inhibitors in Situ

Abstract

No isolation, no purification. An efficient and simple method based on tetrabutylammonium fluoride-mediated alkylation without protecting groups in microtiter plates, followed by screening in situ has been developed to identify new enzyme inhibitors, as demonstrated in the discovery of the shown potent inhibitors of cathepsin B and HIV protease. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2005/z500295_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.