ChemBioChem · 2001 · 31 citations · 0 references
Life SciencesBiochemistryMolecular BiologyCarbohydrate ChemistryGlycobiologyProtein EngineeringCarbohydrate RecognitionCarbohydrate StructureCarbohydrate SynthesisCell-surface GlycansGlycan RecognitionPeptide ChemistryCarbohydrate Receptor EngineeringCarbohydrate ReceptorsCarbohydrate-binding ProteinsPeptide Binding AffinityPeptide Library ScreeningPeptide Ligand DiscoverySialic Acid RecognitionSolid-support Functionalization
We introduced a novel method, through mirror-image phage display, for the identification of high-affinity D-peptides to target specific cell-surface carbohydrates. Both 3-deoxy-alpha-L-manno-2-octulosonic acid (L-KDO) and L-sialic acid and an L-sialo-disaccharide have been synthesized and attached to a solid support for selection of high-affinity peptide binders displayed on phages. Our initial studies in this effort produce single-chain Fab sequences and dodecapeptides that bind to sialic acid and KDO with nanomolar and high micromolar affinity.