Proceedings of the National Academy of Sciences · 1990 · 501 citations · 28 references
Life SciencesCell BiologyMolecular BiologyGene ExpressionTranscriptionTranscriptional RegulationGene Regulatory ElementsInterferon ResponsesPromoter RegulationTranscription FactorsTranscription In EukaryotesTranscriptional Regulation In EukaryotesDna Binding Protein MotifsDna-binding MotifsInterferon-stimulated GenesLigand-dependent RegulationLigand-dependent Transcriptional RegulationTranscription Factor Binding SitesTranscription Factor ComplexesTranscriptional Activator Recruitment
Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.
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