Chemical Communications · 2011 · 67 citations · 21 references
Life SciencesBiophysicsMicrobiologyBiophysical ChemistryMolecular BiophysicsStructural BiologyGlycan StructureMacromolecular AssemblyMacromolecular Assembly ModelingMacromolecular StructureOligosaccharide AssemblyPolysaccharide StructureGlycan Structure AnalysisLectin InteractionsLectin RecognitionLectin-carbohydrate InteractionsLectin-carbohydrate RecognitionLectin-glycan RecognitionLectinsMicrobial Lectins
Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.
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