Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese flounder (Paralichthys olivaceus)

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 181.5K citations

Life Sciences, Molecular Biology, Molecular Genetics +9

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 20.1K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +14

Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets

Claus L. Andersen, Jens Ledet Jensen, Torben F. Ørntoft · Cancer Research · 2004 · 7.2K citations

Life Sciences, Biostatistics, Molecular Biology +17

Guideline to reference gene selection for quantitative real-time PCR

Aleksandar Radonić, Stefanie Thulke, Ian M Mackay et al. · Biochemical and Biophysical Research Communications · 2003 · 1.7K citations

Life Sciences, Genetics, Molecular Biology +9

Housekeeping genes as internal standards: use and limits

Olivier Thellin, Willy Zorzi, Bernard Lakaye et al. · Journal of Biotechnology · 1999 · 1.6K citations · Full text

Life Sciences, Genetics, Molecular Genetics +7