Concepedia

Guideline to reference gene selection for quantitative real-time PCR

Aleksandar Radonić, Stefanie Thulke, Ian M Mackay, Olfert Landt, W. Siegert, Andreas Nitsche

Biochemical and Biophysical Research Communications · 2003 · 1.7K citations · 20 references

DOI

Concepts

References

20

A new mathematical model for relative quantification in real-time RT-PCR

Michael W. Pfaffl · Nucleic Acids Research · 2001 · 34.9K citations · Full text

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 20.1K citations · Full text

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Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

Michael W. Pfaffl · Nucleic Acids Research · 2002 · 7.4K citations · Full text

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Housekeeping genes as internal standards: use and limits

Olivier Thellin, Willy Zorzi, Bernard Lakaye et al. · Journal of Biotechnology · 1999 · 1.6K citations · Full text

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Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR

Thomas D. Schmittgen, Brian A. Zakrajsek · Journal of Biochemical and Biophysical Methods · 2000 · 1.2K citations

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