Journal of Muscle Research and Cell Motility · 2011 · 26 citations · 42 references
Life SciencesCell BiologyMolecular BiologyCell DivisionCell ProliferationCytoskeletonActin FilamentsCell MigrationCytoskeletal DynamicsCytoskeleton DynamicsSecretory PathwayCell Cycle DynamicsFlow Cytometric AnalysisFlow Cytometric Cell AnalysisGolgi Apparatus StructureMyosin FilamentsMyosin MotorsMyosin Regulation
Myosin VI (MVI) is the only known myosin walking towards minus end of actin filaments and is believed to play distinct role(s) than other myosins. We addressed a role of this unique motor in secretory PC12 cells, derived from rat adrenal medulla pheochromocytoma using cell lines with reduced MVI synthesis (produced by means of siRNA). Decrease of MVI expression caused severe changes in cell size and morphology, and profound defects in actin cytoskeleton organization and Golgi structure. Also, significant inhibition of cell migration as well as cell proliferation was observed. Flow cytometric analysis revealed that MVI-deficient cells were arrested in G0/G1 phase of the cell cycle but did not undergo increased senescence as compared with control cells. Also, neither polyploidy nor aneuploidy were detected. Surprisingly, no significant effect on noradrenaline secretion was observed. These data indicate that in PC12 cells MVI is involved in cell migration and proliferation but is not crucial for stimulation-dependent catecholamine release.
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Myosin VI is an actin-based motor that moves backwards
Amber L. Wells, Abel W. Lin, Liqiong Chen et al. · Nature · 1999 · 675 citations