Comparison of three microbial hosts for the expression of an active catalytic scFv

Chaperonin-mediated protein folding at the surface of groEL through a 'molten globule'-like intermediate

Jörg Martin, Thomas Langer, Raina Boteva et al. · Nature · 1991 · 858 citations

Protein Chemistry, Chaperonin-mediated Protein Folding, Protein Assembly +9

Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda

William D. Huse, Lakshmi Sastry, Sheila A. Iverson et al. · Science · 1989 · 805 citations

Engineering, Humoral Response, Immunology +24

Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in <i>Escherichia coli</i>

Kazuyo Nishihara, Masaaki Kanemori, Hideki Yanagi et al. · Applied and Environmental Microbiology · 2000 · 303 citations · Full text

Expression of an antibody fragment at high levels in the bacterial cytoplasm

Pierre Martineau, Peter B. Jones, Greg Winter · Journal of Molecular Biology · 1998 · 216 citations

Antibody Fragment, Bacterial Cytoplasm, Pathogenesis +8

Production of Correctly Folded Fab Antibody Fragment in the Cytoplasm of Escherichia coli trxB gor Mutants via the Coexpression of Molecular Chaperones

Robert B. Weiss, Gang Chen, Brent L. Iverson et al. · Protein Expression and Purification · 2001 · 203 citations

Molecular Chaperones, Protein Assembly, Protein Folding +10