Concepedia

Development of a monoclonal sandwich ELISA for the detection of animal and human Escherichia coli O157 strains

P. Kerr, H. Chart, D. Finlay, D.A. Pollock, D.P. Mackie, H.J. Ball

Journal of Applied Microbiology · 2001 · 43 citations · 37 references

DOI

Abstract

The assay produced is not wholly specific to EHEC O157, but has the potential to be used as a rapid method for screening large numbers of samples for E. coli O157.

References

37

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251K citations

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Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

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[1] Preparation of monoclonal antibodies: Strategies and procedures

G. Galfré, C. Milstein · Methods in enzymology on CD-ROM/Methods in enzymology · 1981 · 1.8K citations

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Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.

Jaime Renart, Jakob Reiser, George R. Stark · Proceedings of the National Academy of Sciences · 1979 · 726 citations · Full text

A Multistate Outbreak of Escherichia coli O157:H7—Associated Bloody Diarrhea and Hemolytic Uremic Syndrome From Hamburgers

Beth P. Bell · JAMA · 1994 · 694 citations