Cloning and Expression of a Yeast Ubiquitin-Protein Cleaving Activity in Escherichia Coli

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

High resolution two-dimensional electrophoresis of proteins.

Patrick H. O’Farrell · Journal of Biological Chemistry · 1975 · 19.3K citations · Full text

Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ

W. David Benton, Ronald W. Davis · Science · 1977 · 4.7K citations

Engineering, Genetics, λGt Recombinant Clones +22

[12] One-step gene disruption in yeast

Rodney Rothstein · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 3.3K citations

Genetics, Yeast, Molecular Genetics +2

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

Stanley Tabor, C C Richardson · Proceedings of the National Academy of Sciences · 1985 · 3.2K citations · Full text

Controlled Exclusive Expression, Bacteriology, Bacteriophage +17