Concepedia

Optimizing cryopreservation of human testicular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants

Victoria Keros, Björn Rosenlund, Kjell Hultenby, Lusine Aghajanova, Lev Levkov, Outi Hovatta

Human Reproduction · 2005 · 208 citations · 31 references

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Abstract

DMSO as a cryoprotectant (at a 0.7 mol/l concentration) proved to maintain the structure of testicular tissue, especially spermatogonia, after cryopreservation better than PrOH or glycerol.

References

31

Histological and Histopathological Evaluation of the Testis

Lonnie D. Russell, Robert A. Ettlin, Amiya P. Sinha Hikim et al. · International Journal of Andrology · 1993 · 2.3K citations

, , +12

Sperm from neonatal mammalian testes grafted in mice

Ali Honaramooz, Amy K. Snedaker, Michele Boiani et al. · Nature · 2002 · 445 citations

, , +8

Transplantation of male germ line stem cells restores fertility in infertile mice

Takehiko Ogawa, Ina Dobrinski, Mary R. Avarbock et al. · Nature Medicine · 2000 · 346 citations · Full text

, , +11

Cryopreservation of human ovarian tissue using dimethylsulphoxide and propanediol-sucrose as cryoprotectants

Outi Hovatta, René Silye, Thomas Krausz et al. · Human Reproduction · 1996 · 338 citations

, , +20

Reconstitution of spermatogenesis from frozen spermatogonial stem cells

Mary R. Avarbock, Clayton J. Brinster, Ralph L. Brinster · Nature Medicine · 1996 · 336 citations

, , +8