Growth at sub-optimal temperatures allows the production of functional, antigen-binding Fab fragments in Escherichia coli

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

James S. Huston, Dennis J. Levinson, M Mudgett-Hunter et al. · Proceedings of the National Academy of Sciences · 1988 · 1.6K citations · Full text

Engineering, Biochemistry, Molecular Biology +17

Single-Chain Antigen-Binding Proteins

Robert E. Bird, Karl D. Hardman, James W. Jacobson et al. · Science · 1988 · 1.5K citations

Pharmacy, Biochemistry, Molecular Biology +13

Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Jeffry J. Leary, David J. Brigati, David C. Ward · Proceedings of the National Academy of Sciences · 1983 · 1.1K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +14

Assembly of a Functional Immunoglobulin F _v Fragment in <i>Escherichia coli</i>

Arne Skerra, Andreas Plückthun · Science · 1988 · 969 citations

Life Sciences, Biochemistry, Molecular Biology +17

<i>Escherichia coli</i> Secretion of an Active Chimeric Antibody Fragment

Marc Better, Chun‐Wei Chang, Randy Robinson et al. · Science · 1988 · 575 citations

Pharmacy, Biochemistry, Molecular Biology +15