Biotechnology and Bioengineering · 2006 · 58 citations · 14 references
Life SciencesMicrobiologyMolecular BiologyBiosensorsMolecular VirologyVirologyAntigen Detection AssaysHost-pathogen RecognitionInfectious Disease DetectionPathogen DetectionPathogen RecognitionViral InfectionsPlasmonic BiosensorsResponse SpecificitySurface Plasmon Resonance AnalysisSurface Plasmon Resonance BiosensorsViral Antigen DetectionViral Disease DiagnosisViral Insect PathogensVirus Detection Biosensors
Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors.
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Detection of foodborne pathogens using surface plasmon resonance biosensors
V. Koubová, Eduard Brynda, L Karasová et al. · Sensors and Actuators B Chemical · 2001 · 286 citations