Elaboration of an electroporation protocol for large plasmids and wild-type strains of<i>Bacillus thuringiensis</i>

Abstract

The present study established an effective electroporation protocol for large plasmids and wild type strains of B. thuringiensis. This method is well suited for the cloning and expression of huge DNA fragments such as gene clusters in B. thuringiensis. It also can be used as a reference method for other Bacillus strains that are refractory to electroporate.