Mutational analysis of the RNA pseudoknot component of a coronavirus ribosomal frameshifting signal

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.1K citations

Genetics, Puc19 Vectors, Bacteriophage +9

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.8K citations · Full text

Genetics, Bacteriology, Bacteriophage +18

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

Engineering, Reverse Genetics, Efficient Site-specific Mutagenesis +14

Functional messenger RNAs are produced by SP6<i>in vitro</i>transcription of cloned cDNAs

Paul A. Krieg, Douglas A. Melton · Nucleic Acids Research · 1984 · 1.5K citations · Full text