Immunology · 2000 · 207 citations · 27 references
Life SciencesImmunologyPathologyImmunology Of Infectious DiseasesInflammationInnate ImmunityAntigen RecognitionCytokine BiologyImmune-mediated Inflammatory DiseasesNeutrophil BiologyT Cell ActivationT Cell BiologyAntigen Presenting Cell Costimulatory MoleculesMajor Histocompatibility Complex Class Ii Antigen ProcessingMajor Histocompatibility Complex Class Ii PresentationMhc Class Ii MoleculesNeutrophil InvolvementT Cell Activation CytokinesT Cell-mediated InflammationT-cell Mediated Inflammation
Polymophonuclear cells (PMN) of healthy donors do not express major histocompatibility complex (MHC) class II antigens or the T-cell costimulatory molecules CD80 or CD86. Expression of these receptors, however, is seen in patients with chronic inflammatory diseases. We now report that, by culturing PMN of healthy donors with autologous serum, interferon-gamma (IFN-gamma) and granulocyte-macrophage colony-stimulating factor (GM-CSF), de novo synthesis of MHC class II, CD80 and CD86 could be induced. MHC class II-positive PMN acquired the capacity to present staphylococcus enterotoxin to peripheral T cells, apparent as induction of interleukin-2 (IL-2) synthesis and proliferation of the T cells. Moreover, the PMN also processed tetanus toxoid (TT) and induced proliferation of TT-specific T cells in a MHC class II-restricted manner. Taken together, these data indicate that PMN can be activated to function as accessory cells for T-cell activation.
27
Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products
Francesco Colotta, Fabio Re, Nadia Polentarutti et al. · Blood · 1992 · 1.1K citations