Journal of the Institute of Brewing · 2005 · 29 citations · 28 references
Grain ProtectionEngineeringFungal Cell BiologyGeneticsPlant PathologyCrop ImprovementGrain QualityBiosynthesisGrain ScienceYeastPlant Pathogen EffectorFusarium GrowthBiochemistryAlternative Protein SourceAgricultural BiotechnologyPlant Pathogen FusariumFungal PathogenBarley Grain Ns-ltpBiomolecular EngineeringPlant ImmunityCrop ProtectionCrop ScienceBiotechnologyGenetic EngineeringSeed StorageMicrobiologyHost ResistanceMedicineGrain Storage
A basic protein (pI < 9) was isolated to homogeneity from a domestic cultivar of malting barley grain (Hordeum vulgare). In its unreduced form it exists as a dimer of a 9 kDa protomer with four disulphide bridges. These characteristics together with protein sequence data revealed that the isolated protein belongs to the class of ns-LTP. The antifungal potency of malting barley grain ns-LTP was examined on Saccharomyces cerevisiae, Candida albicans and the plant pathogen Fusarium solani growth in vitro. It was found that ns-LTP inhibits Saccharomyces and Fusarium growth; the concentration required for 50% inhibition after 24 h of incubation (IC50) was 100 and 80 μg/mL, respectively. On the basis of these results, the importance of ns-LTP for barley grain protection from fungal diseases has been discussed.
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