The composition of the difficultly extractable soybean phosphatides

Abstract

Summary The object of the present work has been to study those soybean phosphatides which cannot be extracted by means of nonpolar solvents but only be means of mixtures of nonpolar and polar solvents, for instance hexane and ethanol. These phosphatides were fractionated by the countercurrent distribution technique, and the following groups of substances were found: a) carbohydrates with d‐inositol in the form of the methyl ether called pinitol; b) a number of nitrogen‐containing substances, the nature of which is not as yet fully elucidated but which is perhaps merely decomposition products of proteins; c) a glyceroinositophosphatidic acid which contains equimolar quantities of glycerophosphoric acid and inositolmonophosphoric acid and phosphorus and fatty acids at a ratio of about 1 to 2; d) a high‐molecular phosphatide acid; and e) a mixture of the three glycerophosphatides: phosphatidylcholine,‐ethanolamine, and‐serine.