The Use of Microtiter Plates in Mixed Lymphocyte Cultures

Abstract

Experiments were performed to develop a lymphocyte culture system in which microtiter plates could be used as a practical and time‐saving technique. The suitability of different types of microtiter plates was tested, analyzing lymphocyte reactivity by means of thymidine incorporation after stimulation by PHA and in the MLG. Furthermore, it became clear that in this system the number of lymphocytes required per culture could be diminished. According to a Workshop program (this issue), some experiments were carried out to further clarify the reproducibility and reliability of this culture system.