Transplantation · 1971 · 354 citations · 0 references
Life SciencesHematologyImmunologyCellular ImmunologyClinical ImmunologyImmunogeneticsCell CultureHistocompatibilityHistocompatibility AntigensHla MatchingHla TypingMajor Histocompatibility ComplexHla Compatibility AssessmentHla Compatibility TestingHla-dr MatchingHuman Leukocyte AntigensLeukocyte PopulationsMajor Histocompatibility Complex MatchingMixed Lymphocyte Reaction
A miniaturized mixed leukocyte culture test is presented that reduces cell, medium, and time requirements. The assay is performed in 0.2‑ml microtiter plates, with responder and stimulator cell concentrations adjusted per experiment. The miniaturized assay discriminates isogeneic from allogeneic mixtures over a 16–20‑hour window beginning at 48 hr and yields MLC identity measurements that correlate strongly with the conventional macro method.
A miniaturized method for the mixed leukocyte culture (MLC) test is described which results in substantial savings in cells, medium, and time. The test is done in microtiter plates using 0.2-ml culture volumes. Concentrations of responding and stimulating cells are varied, depending on the experiment. Significant discrimination between isogeneic and allogeneic mixtures is possible when cultures are labeled for a 16–20-hr period starting at 48 hr. The method measures MLC identity, as previously defined, and quantitates in a manner significantly correlated with the macro method.