Proceedings of the National Academy of Sciences · 1995 · 359 citations · 33 references
Life SciencesBiochemistryCell BiologyCell ProliferationCytoskeletonSignal TransductionCytoskeletal ArchitectureCytoskeletal Associated ProteinsCytoskeletal RegulationMicrotubule-associated ProteinsMitogenic SignalingProtein Kinase CascadesBiochemical CharacterizationBiochemical FractionationMapk SignalingMitogen SignalingMitogen-activated Protein Kinase CascadesMitogen-activated Protein Kinase KinaseMitogen-activated Protein Kinase PathwaysMitogen-activated Protein Kinase Signaling
Using indirect immunofluorescence microscopy and biochemical techniques, we have determined that approximately one-third of the total mitogen-activated protein kinase (MAPK) is associated with the microtubule cytoskeleton in NIH 3T3 mouse fibroblasts. This population of enzyme can be separated from the soluble form that is found distributed throughout the cytosol and is also present in the nucleus after mitogen stimulation. The microtubule-associated enzyme pool constitutes half of all detectable MAPK activity after mitogenic stimulation. These findings extend the known in vivo associations of MAPK with microtubules to include the entire microtubule cytoskeleton of proliferating cells, and they suggest that a direct association of MAPK with microtubules may be in part responsible for the observed correlations between MAPK activities and cytoskeletal alteration.
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The stress-activated protein kinase subfamily of c-Jun kinases
John Kyriakis, Papia T. Banerjee, Eleni Nikolakaki et al. · Nature · 1994 · 2.6K citations