Biotechnology and Bioengineering · 1991 · 136 citations · 16 references
Life SciencesBiochemistryBiotechnologyBiocatalysisEnzymologyFermentation EngineeringAlcoholic FermentationBiofuel ProductionGlycan DegradationSugar ProcessingCellulase ActivityCellulase EnzymesCellulase TreatmentEnzymatic HydrolysisLignocellulosic Biomass ConversionLignocellulosic Biomass PretreatmentSaccharificationSteam Explosion
Effects of time, temperature, and pH during the steam explosion of poplar wood were studied with the aim of optimize both pentoses recovery and enzymatic hydrolysis efficiency. Steam explosion of acid impregnated wood chips allowed the recovery of 70% of potential xylose as monomers (217 degrees C, 120 s) Enzymatic hydrolysis of pretreated fiber with Trichoderma reesei CL-847 cellulase system increased progressively with the severity of the steam treatment conditions. The best yield in term of glucose recovery after 24 h of enzymatic hydrolysis was 70% of potential glucose (225 degrees C, 120 s). Deactivation by adsorption on lignin of Trichoderma reesei cellulases and inhibition of these enzymes by low-molecular-weight phenols and trihydroxybutyric acids were noticed.
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