Electrophoretic Characterization of Bovine Lipoprotein Subfractions Isolated by Agarose Gel Chromatography

Abstract

Lipoproteins isolated from whole bovine serum were applied to agarose gel columns and fractionated by molecular size. Three distinct lipoprotein absorbance peaks (280 nm) emerged from the columns (Peaks 1, 1I, and III). A typical elution profile exhibited lipoprotein distribution of approximately 11% Peak I, 6% Peak II, and 83% Peak III. Characterization of Peak 1 lipoproteins on agarose gel electrophoresis and polyacrylamide gel electrophoresis suggested that Peak I consisted of very low density lipoproteins.