PURIFICATION OF PEPTIDES: AN EFFICIENT PROCEDURE FOR THE SEPARATION OF PEPTIDES FROM AMINO ACIDS AND SALT

Abstract

A novel method for the separation of peptides from amino acids and salts has been developed. Separation is achieved by passage of the sample through a column of octadecasilyl-silica (ODS). Peptides, but not amino acids and salts, are strongly retained by ODS in an aqueous mobile phase and can be recovered by elution with an aqueous-alcoholic mobile phase. The method is applicable to most peptides and is fast and simple. Moreover, it is characterized by good recovery yields and high sample capacity.