Analysis of the aphthovirus 2A/2B polyprotein ‘cleavage’ mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal ‘skip’

Michelle Donnelly, Garry A. Luke, Amit P. Mehrotra, +4

Journal of General Virology

Versatile retroviral vectors for potential use in gene therapy.

Robert G. Hawley, F H Lieu, A Z Fong, +1

PubMed

Betaine Improves the PCR Amplification of GC-Rich DNA Sequences

Wolfgang Henke, K. Herdel, Klaus Jung, +2

Nucleic Acids Research

GeneticsSecondary StructureDna AnalysisMolecular BiologyNucleic Acid Amplification Test +14

Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein.

Martin D. Ryan, Jeff Drew

The EMBO Journal

Mediated CleavageVirus StructureMolecular VirologyPathogenesisImmunology +5

Betaine can eliminate the base pair composition dependence of DNA melting

William A. Rees, Thomas D. Yager, John Korte, +1

Biochemistry

Retroviral-Mediated Transfer of the Green Fluorescent Protein Gene Into Murine Hematopoietic Cells Facilitates Scoring and Selection of Transduced Progenitors In Vitro and Identification of Genetically Modified Cells In Vivo

Derek A. Persons, James A. Allay, Esther R. Allay, +4

Blood

Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases

Kulandaiappan Varadaraj, Dorothy M. Skinner

Gene

Polymerase Chain ReactionEngineeringPcr AmplificationMolecular Biological MethodGenetics +11

Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

Namadev Baskaran, Rajendra P. Kandpal, A.K. Bhargava, +3

Genome Research

Deoxyribonucleic AcidsFragile X RegionGeneticsDna AnalysisMolecular Biology +18

The timing of TCRα expression critically influences T cell development and selection

Troy A. Baldwin, Michelle M. Sandau, Stephen C. Jameson, +1

The Journal of Experimental Medicine

Rapid analysis of T-cell selection in vivo using T cell–receptor retrogenic mice

Jeff Holst, Kate M. Vignali, Amanda R. Burton, +1

Nature Methods

Adaptive Immune SystemImmune RegulationImmunologyImmunodominanceT Cell Immunity +7