Determination of mono‐, di‐, and triglycerides by molecular distillation and thin‐layer chromatography

Abstract

Abstract Analysis of mixtures of mono‐, di‐, and triglycerides by molecular distillation and thin‐layer chromatography is described. Mono‐ and diglycerides undergo appreciable acyl migration through the effect of heat during molecular distillation. Nevertheless this technique may be used for the quantitative analysis of mixtures of mono‐, di‐, and triglycerides, provided there are no substances present which catalyze, disproportionation. Thin‐layer chromatographic (TLC) analysis of mono‐, di‐, and triglycerides is fast and simple and can be carried out on a micro‐scale with a high degree of accuracy and precision. It also is extremely sensitive, permitting the quantitative estimation of as little as 0.1% of a single component in a mixture.