Evaluation of antioxidants by a rapid polarographic method

Abstract

Abstract A simple, rapid, precise, and reproducible method and an adaptable apparatus for polarographic measurement of lipid peroxidation and antioxidant activity is described. This method measures antioxidant activity within a few min time as compared to the hours or days needed for several widely used methods. Peroxidation rates were measured for different lipids as a function of amount of lipid, and amount of biocatalyst. The method is applicable to the detection and measurement of the antioxidant effects exhibited by weak antioxidants and has been satisfactorily used for the measurement of the antioxidant activity of different concentrations of powerful antioxidants.