Audiology and Neurotology · 2009 · 45 citations · 30 references
Hair CellsVestibular Hair CellsTranslational MedicineVector Delivery MiceAllergyImmunotoxicologyMedicineImmunologyCell DeathImmunotherapyPharmacologyCell BiologyCell SignalingOuter Hair CellsAuditory Hair Cells
To evaluate the protective effects of bcl-2, we have developed an in vivo model of gentamicin ototoxicity in C57BL/6 mice using intratympanic delivery of gentamicin. Hair cell survival was evaluated using myosin VIIa immunohistochemistry, cytocochleogram and auditory brainstem response (ABR) testing. At 10 days after gentamicin application, a consistent loss of outer hair cells was seen. Mice were pretreated with an adenovector expressing human bcl-2 (Ad.11D.bcl-2) or a control vector (Ad.11D). Seventy-two hours after vector delivery mice were treated with intratympanic gentamicin and evaluated at 10 days after ototoxin delivery. Pretreatment with Ad.11D.bcl-2 resulted in morphologic protection of hair cells and preservation of hearing thresholds measured by ABR.
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Bcl-2 inhibition of neural death: decreased generation of reactive oxygen species
Darci J. Kane, Theodore A. Sarafian, Rein Anton et al. · Science · 1993 · 1.7K citations
Neural Death, Apoptosis, Immunology +21