Journal of Analytical Methods in Chemistry · 1991 · 21 citations · 2 references
Protein ChemistryEngineeringBiochemistryProtein FoldingBioenergeticsBioanalysisMicrowave EnergyBiochemical EngineeringBiotechnologyDegrees C. HydrolysisProtein PurificationMedicineEnzymatic ModificationMicrowave SynthesisBiophysicsBiomolecular EngineeringComplete Hydrolysis
Microwave energy, at manually-adjusted, partial power settings has been used to hydrolyse bovine serum albumin at 125 degrees C. Hydrolysis was complete within 2 h, except for valine and isoleucine which were completely liberated within 4 h. The aminoacid destruction was less than that observed at similar hydrolysis conditions with other methods and complete hydrolysis was achieved more rapidly. These results provide a basis for automating the process of amino-acid hydrolysis.
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Handbook of Chemistry and Physics.
Annals of Internal Medicine · 1973 · 2.6K citations