An improved technique for the efficient construction of gene libraries by partial filling-in of cohesive ends

A general method for isolation of high molecular weight DNA from eukaryotes

Nikolaus Blin, Darrel W. Stafford · Nucleic Acids Research · 1976 · 2.8K citations · Full text

Life Sciences, Genetics, Molecular Biology +14

Lambda replacement vectors carrying polylinker sequences

Anna‐Maria Frischauf, Hans Lehrach, Annemarie Poustka et al. · Journal of Molecular Biology · 1983 · 1.8K citations

Life Sciences, Genetics, Molecular Biology +7

Size fractionation of double-stranded DNA by precipitation with polyethylene glycol

John T. Lis, Robert Schleif · Nucleic Acids Research · 1975 · 239 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +10

Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.

Steven B. Zimmerman, Barbara H. Pheiffer · Proceedings of the National Academy of Sciences · 1983 · 145 citations · Full text

Life Sciences, Molecular Biology, Molecular Biophysics +11

Representation of DNA sequences in recombinant DNA libraries prepared by restriction enzyme partial digestion

Brian Seed, Richard C. Parker, Norman Davidson · Gene · 1982 · 90 citations

Life Sciences, Genetics, Molecular Biology +13