Nucleic Acids Research · 1976 · 2.8K citations · 8 references
Life SciencesGeneticsMolecular BiologyDna RepairMicrobial GeneticsNucleic Acid BiochemistryDna ChemistryDna StructureNucleic Acid ExtractionNucleic Acid PurificationCell-free Nucleic Acid IsolationDna ExtractionDna Fragment IsolationDna Single-strand BreaksGenomic Dna PurificationSingle-strand Dna BreaksTissue Nucleic Acid Extraction
A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.
8
Sedimentation studies of the size and shape of DNA
F. William Studier · Journal of Molecular Biology · 1965 · 2.2K citations
Procedures in Nucleic Acid Research.
Waldo E. Cohn · Journal of the American Chemical Society · 1967 · 2K citations