Journal of Agricultural and Food Chemistry · 2010 · 14 citations · 38 references
Life SciencesCell BiologyPharmacologyCell JunctionsCell SignalingSignal TransductionCell-cell JunctionsGap Junction CommunicationGap JunctionsIntercellular CommunicationJunctional ComplexesMammary Epithelial Cell BiologyAltered Intercellular CommunicationConnexin 43 SignalingExtracellular Signal-regulated Kinase PathwayExtracellular Signal-regulated Kinase SignalingGap Junction Intercellular CommunicationMammary Epithelial Cell FunctionPhosphorylation RegulationReactive Oxygen Species Generation
The protective effect of c9,t11-conjugated linoleic acid (CLA) on the inhibition of gap junctional intercellular communication (GJIC) was examined in a human mammary epithelial cell line (MCF-10A) treated with 12-O-tetradecanoylphorbol-13-acetate (TPA), relative to t10,c12-CLA isomer. TPA inhibited GJIC in a dose-dependent and reversible manner and was associated with connexin 43 phosphorylation. Pretreatment of 20 μM c9,t11-CLA for 24 h prior to 60 nM TPA for 1 h prevented the inhibition of GJIC by reducing the phosphorylation of connexin 43 via suppressing extracellular signal-regulated kinases (ERK1/2) activation. Reactive oxygen species (ROS) accumulation by TPA was attenuated by c9,t11-CLA. The efficacy of c9,t11-CLA in protecting inhibition of GJIC, connexin 43 phosphorylation, and ROS production was superior to that of t10,c12-CLA. These results suggest that c9,t11-CLA, including t10,c12-CLA, prevents the carcinogenesis of MCF-10A cells by protecting down-regulation of GJIC during the cancer promotion stage, and lack of their toxicities could be an excellent indicator for the chemoprevention of breast cancer.
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Dorothee C. Schönwaßer, Richard Marais, Christopher J. Marshall et al. · Molecular and Cellular Biology · 1998 · 760 citations · Full text
Mammary cancer prevention by conjugated dienoic derivative of linoleic acid.
C Ip, S.F. Chin, J A Scimeca et al. · PubMed · 1991 · 743 citations