Chemistry in Living Cells: Detection of Active Proteasomes by a Two‐Step Labeling Strategy

Abstract

In vivo targeting of the proteasome: Probe 1 is a cell-permeable irreversible inhibitor that alkylates the active-site residues of the proteasome in a Michael fashion. After cell lysis, a biotin moiety is introduced by Staudinger ligation to yield construct 2. This strategy allows activity profiling of the catalytic activities of the proteasome in vivo. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2003/z51314_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.