Lab on a Chip · 2012 · 100 citations · 20 references
EngineeringImmunologyOrgan-on-a-chipBiomedical EngineeringCellular PhysiologyInflammationVivo-like Transendothelial MigrationMicrofluidicsLaboratory MedicineBiofluid DynamicBiophysicsGranulocyteCell TraffickingInflammatory ProcessInflammatory ResponseBiomedical AnalysisVascular BiologyPhagocyteMicrofluidic DeviceVersatile AssayBiomedical ImagingEndothelial DysfunctionCell MigrationBiomemsMedicineExtracellular Matrix
Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.
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Rapid leukocyte migration by integrin-independent flowing and squeezing
Tim Lämmermann, B. Bader, Susan J. Monkley et al. · Nature · 2008 · 1.4K citations