A rapid and simple method for reversing the inhibitory effect of heparin on PCR for HLA class II typing.

Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material

P. Sean Walsh, David A. Metzger, Russell Higuchi · BioTechniques · 2013 · 5.9K citations · Full text

Engineering, Dna Analysis, Molecular Biology +22

Interference of heparin with the polymerase chain reaction.

Ernest Beutler, Terri Gelbart, Willi Kuhl · PubMed · 1990 · 278 citations

Polymerase Chain Reaction, Protein Expression, Molecular Biology +2

Use of Chelex to improve the PCR signal from a small number of cells

J. Singer-Sam · Medical Entomology and Zoology · 1989 · 266 citations

Multiplex Technology, Bioanalysis, Molecular Biology +6

Inhibition of human immunodeficiency virus gene amplification by heparin

Mark Holodniy, Su Yeon Kim, David Katzenstein et al. · Journal of Clinical Microbiology · 1991 · 197 citations · Full text

Acid Citrate Dextrose, Gene Amplification, Biochemistry +15

Immunoaffinity-purified DNA polymerase .alpha. displays novel properties

Heinz‐Peter Nasheuer · Biochemistry · 1987 · 87 citations