Proceedings of the National Academy of Sciences · 1979 · 41 citations · 12 references
Crystal StructureImmunocytochemical TechniqueStructural BioinformaticsGlycobiologyMolecular BiologyKappa FabProtein X-ray CrystallographyImmunochemistryGlycosylationProtein FunctionBiochemistryCell BiologyStructural BiologyMouse ImmunoglobulinNatural SciencesImmunoglobulin EIndividual Immunoglobulin DomainsCellular BiochemistryMedicine4.5-A ResolutionCarbohydrate-protein Interaction
An electron-density map of the mouse galactan-binding immunoglobulin J539 (IgA2,kappa) Fab has been calculated to a resolution of 4.5 A by the method of heavy atom isomorphous replacement with four derivatives. The map has been interpreted with the aid of a computer program which systematically searched for the best fit between the electron-density map and the known coordinates of individual immunoglobulin domains. The quaternary structure of J539 Fab at this resolution appears similar to that of another mouse immunoglobulin, IgA2,kappa Fab, McPC603. The model coordinates for J539 Fab should allow us to proceed directly to a high-resolution structure determination without further heavy atom isomorphous replacement.
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David M. Segal, Eduardo A. Padlan, Gerson H. Cohen et al. · Proceedings of the National Academy of Sciences · 1974 · 465 citations · Full text
Mcpc 603, Proteinlipid Interaction, Immunocytochemical Technique +20